A perfusion culture system for virus vaccine manufacture in diploid cell cultures.

Optimization of Microcarrier-based MDCK-SIAT1 Culture System for Influenza Virus Propagation

  Introduction: The preparation of seasonal influenza virus vaccines and especially its large-scale production requirement after the emergence or reemergence of a pandemic will need an alternative host cell system due to current suboptimal methods and the insufficiency of embryonated chicken eggs needed for producing them. In response to the vital and increasing demand for alternative means for ...

Production of Rabies Vaccine Using BHK-21 with Roller Bottle Cell Culture Technique

Compared to other methods of vaccine production, (e.g. culture flask and bioreactor system), the roller culture method for producing rabies vaccine is simpler, and the equipment easier to install and maintain, and also in the event of contamination of one roller, the entire batch need not be discarded. This system provides a much larger surface area than the culture flask, and also ensures a mu...

Development of a diploid cellline from fetal calf lung for virus vaccine production

Evaluation and Optimization of Chick Embryo Fibroblasts for Production of a Fowl Pox Vaccine Based on Cell Culture

Abstract Background and Aims: For more than half a century, the production of fowl pox vaccine at Razi Vaccine and Serum Research Institute, has been carried out by injection method in the chick chorioallantoic membrane (CAM) and the vaccine has a favorable and effective in poultry flocks and has provided a complete satisfaction to the poultry flocks owner. Fowl pox vaccine is also manufactured...

Replication Kinetic of Infectious Laryngotracheitis Virus in Embryonic Chicken Neural Stem Cell

Background and Aims: Infectious laryngotracheitis virus (ILTV) infection causes a highly contagious respiratory disease in poultry leads to significant economic losses in the poultry industry. The genomic and antigenic characteristics between virulent and vaccine strains of ILTV are very similar. The ability of embryonated chicken neural stem (CNS) cell for differential diagnosis of ILTV strain...